Ozone application for the people health state monitoring by the total unsaturation index determination

There exists evidence about the influence of oxidative stress in certain pathologic states, especially chronic diseases. Measurements of lipid peroxide levels and the levels of their byproducts are routinely used as indices of oxidative stress; however, until now, analytic assays used to determine lipid peroxidation have limitations regarding sensitivity, specificity, and timing of analysis. In the present brief review, we introduce the method of the double bond index (DB-index), a promising method of evaluation for lipid metabolism disturbance and lipid peroxidation measurement. DB-index calculation is based on the patented Total Unsaturation Analyzer (TUA) which instrumentation is fundament on the organic unsaturated compounds property of integrate ozone in the double bond site (one ozone molecule by each double bond) in a fast way. By this technique it is possible to determine in a short time (1-3 minutes) and with high precision (± 1%) the lipids total unsaturation in plasma and in cellular membranes. Some notable cases where DB-index has been established as diagnostic and prognostic criteria in different diseases and possibilities of present and future applications in the clinical analysis are discussed.